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Related Experiment Videos

Impaired IgE response in SWAP-70-deficient mice.

T Borggrefe1, S Keshavarzi, B Gross

  • 1Basel Institute for Immunology, Basel, Switzerland.

European Journal of Immunology
|August 14, 2001
PubMed
Summary
This summary is machine-generated.

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SWAP-70 protein deficiency in mice increases B cell sensitivity to radiation and autoantibody development. This protein plays a specific role in the CD40 signaling pathway, particularly impacting the IgE immune response.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • SWAP-70 protein is found in activated B cells and is involved in immunoglobulin class switching.
  • SWAP-70 translocates to the nucleus post-B cell activation, suggesting a role in nuclear signaling.
  • The specific function of SWAP-70 in immune responses remained largely uncharacterized.

Purpose of the Study:

  • To investigate the in vivo function of SWAP-70.
  • To determine the role of SWAP-70 in B cell responses, DNA damage sensitivity, and autoantibody production.
  • To elucidate SWAP-70's involvement in the CD40 signaling pathway and immunoglobulin isotype switching.

Main Methods:

  • Generation and analysis of SWAP-70-deficient mice.
  • Assessment of B lymphocyte sensitivity to gamma-irradiation.

Related Experiment Videos

  • Evaluation of autoantibody levels and immunoglobulin class switching (IgE, other isotypes).
  • Analysis of CD40 and LPS signaling pathways in mutant mice.

    • Main Results:

    • SWAP-70-deficient B cells exhibit 2-3 fold increased sensitivity to gamma-irradiation.
    • SWAP-70-deficient mice show a higher frequency of autoantibody development.
    • CD40-dependent IgE class switching is significantly reduced (5-8 fold in vitro), with lower IgE levels in vivo.
    • CD40-induced proliferation is transiently increased, while LPS-induced responses are normal.

    Conclusions:

    • SWAP-70 plays a critical role in the CD40 signaling pathway, specifically regulating IgE class switching.
    • SWAP-70 deficiency impacts B cell DNA damage response and immune tolerance, leading to autoimmunity.
    • The findings highlight SWAP-70 as a key regulator in adaptive immunity, particularly in IgE-mediated responses.