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Updated: Oct 5, 2026
A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth
Published on: December 22, 2020
[Experimental study of biologic effects of tumour necrosis factor-alpha on scar-derived fibroblasts]
1Department of Plastic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, PR China.
Objective:
To investigate the biologic effects of tumour necrosis factor-alpha(TNF-alpha) on scar-derived fibroblasts and to evaluate the role of TNF-alpha in genesis, prevention and treatment of scar.
Methods:
Cell strains were established by tissue culture technique. To study the fibroblasts derived from hypertrophic scar and normal dermis, cytoactivity and expression of fibronectin (FN) were determined by MTT, FCM and immunofluorescence.
Results:
1. When TNF-alpha is at a high concentration (1,000 U/ml increases), the cytoactivity of hypertrophic scar fibroblasts was decreased significantly, and the positive rate of FN expression was decreased by 11.7%. 2. Cytoactivity of normal dermis fibroblasts was increased with TNF-alpha while the positive rate of FN expression was increased by 9.6%.
Conclusions:
TNF-alpha has different biological effects on fibroblasts derived from hypertrophic scar or normal dermis. Studying the regulative mechanisms of TNF on abnormal scar will probably provide a new way to prevent and cure it.
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