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[Study on the expression of calreticulin in hypertrophic scar-derived fibroblasts]
Objective:
To investigate the expression level and intracellular distribution of calreticulin(CRT) in hypertrophic scar-derived fibroblasts(HSF) and normal skin-derived fibroblasts(NSF).
Methods:
Using anti-CRT antibody, CRT was detected in HSF and NSF by immunocytochemistry and ELISA technique.
Results:
CRT was present in the cytoplasm and nuclei in proliferating HSF or NSF in culture, but HSF possessed significantly higher expression level of CRT than NSF(P < 0.01). When these cells (HSF and NSF) were fused, neither intracellular nor intranuclear staining for CRT was observed.
Conclusion:
In HSF and NSF, the distribution of CRT is widespread. Because of its abundant expression in HSF, we postulate that CRT as a multifunctional protein plays an important role in Ca2+ sequestering, integrin-mediated signalling and cell adhesion.