Determination of moclobemide, paroxetine, and fluvoxamine in tablets by HPLC

A simple, accurate high performance liquid chromatography procedure is presented in order to determine moclobemide in Aurorix-tablets, paroxetine in Seroxat-tablets and fluvoxamine in Fevarin tablets. These drugs were chromatographed on a Nova-Pak C18, (dp = 4 microm), 150x3.9 mm i.d. column using methanol/phosphate butter adjusted to pH 2.65 with phosphoric acid (7:3, v/v) as the mobile phase to determining of the moclobemide and methanol/tetrahydrofuran/phosphate buffer at pH 2.65 (53:5:4, v/v) for determining of paroxetine and fluvoxamine. The detection was carried at 235 nm. The method was tested for linearity over the range 5-50 microg/ml. The relative standard deviation for moclobemide is 1.16, but for paroxetine and fluvoxamine is less than 1% (0.25 and 0.46 respectively).