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Proposed phytic acid standard including a method for its analysis.

S P Brooks1, D Oberleas, B A Dawson

  • 1Health Canada, Nutrition Research Division, Banting Research Centre, Ottawa, ON.

Journal of AOAC International
|August 15, 2001
PubMed
Summary
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A new method accurately quantifies phosphorous in phytic acid, enabling its use as a food analysis standard. This rapid technique requires minimal sample and is completed in three hours.

Area of Science:

  • Analytical Chemistry
  • Food Science

Background:

  • Phytic acid is a major phosphorus storage compound in plant seeds.
  • Accurate quantification of phytic acid is crucial for food analysis and nutritional studies.

Purpose of the Study:

  • To develop and validate a rapid and accurate method for quantifying free and total phosphorous in purified phytic acid.
  • To establish phytic acid as a reliable standard for phytic acid determinations in food matrices.

Main Methods:

  • The method involves a wet ashing procedure for sample digestion.
  • Phosphorous content is measured using a 1-amino-2-naphthol-4-sulfonic acid-molybdate reagent.
  • Measurements are conducted using a microplate reader at a wavelength of 660 nm.

Main Results:

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  • The developed method accurately quantifies both free and total phosphorous in a commercial phytic acid preparation.
  • The procedure is rapid, with a total analysis time of approximately 3 hours.
  • The method requires a minimal sample amount, as little as 50 mg.

Conclusions:

  • The described method provides a reliable and efficient means for phytic acid analysis.
  • This validated method facilitates the use of purified phytic acid as a standard in food analysis.
  • The technique's speed and low sample requirement make it suitable for routine laboratory applications.