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Updated: Jul 17, 2026

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High-Throughput Measurement and Classification of Organic P in Environmental Samples
Published on: June 8, 2011
Proposed phytic acid standard including a method for its analysis
S P Brooks1, D Oberleas, B A Dawson
1Health Canada, Nutrition Research Division, Banting Research Centre, Ottawa, ON.
Journal of AOAC International
|August 15, 2001
Abstract:
A method is described that accurately and rapidly quantifies the free and total phosphorous content of a commercially available, purified, phytic acid preparation. This allows its use as a standard for phytic acid determinations in foods. The method involves a wet ashing step followed by phosphorous measurement with a 1-amino-2-naphthol-4-sulfonic acid-molybdate reagent in a microplate reader at 660 nm. The procedure can be performed in 3 h with as little as 50 mg sample.

