Quantification of TGF-beta1 mRNA along rat nephron in obstructive nephropathy

Unilateral ureteral obstruction (UUO) leads to interstitial fibrosis of the obstructed kidney, and transforming growth factor-beta1 (TGF-beta1) is thought to play an important role in this process. Although increased TGF-beta1 mRNA expression in the obstructed kidney has been demonstrated, the source of the increased TGF-beta1 remains to be elucidated. To determine the precise localization of TGF-beta1 in the obstructed kidney, we examined TGF-beta1 mRNA expression using in situ hybridization and competitive RT-PCR in rats with UUO. In situ hybridization demonstrated that TGF-beta1 mRNA expression was preferentially increased in tubular epithelial cells and to a lesser degree in infiltrating macrophages in obstructed kidneys. Quantitative analysis using competitive RT-PCR in microdissected nephron segments revealed that levels of TGF-beta1 mRNA in obstructed kidneys relative to control kidneys increased significantly in proximal tubules, thick ascending limbs of Henle, and distal convoluted tubules, whereas those in glomeruli and collecting ducts did not change significantly. Of the tubular segments, the proximal tubules appeared to predominantly contribute to increased TGF-beta1 mRNA. Our findings suggest that renal tubules, particularly proximal tubules, are the main contributors to increased TGF-beta1 mRNA expression in obstructed kidneys and to the subsequent interstitial fibrosis.