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[Molecular design and immunogenicity studies of multiple antigenic peptide corresponding to envelope glycoprotein
1Department of Infectious Disease of Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Objective:
To study the immunogenicity of multiple antigen peptide (MAP) corresponding to the hypervariable region 1 (HVR1) within the putative envelope glycoprotein E2/NS1 of hepatitis C virus (HCV).
Methods:
Basing upon the E2/NS1 consensus sequence of HCV-BJ (isolate of Beijing) which we had obtained previously, and referring to several HCV HVR1 sequences reported by foreign authors, we synthesized a linear epitope peptide (LP) and a MAP (symmetric 8 branches) according to 22 amino acids (position 390 - 411 aa) of HCV-BJ HVR1 on the basis of our prediction to their antigenicity parameters. Then, the Balb/C mice and rabbits were immunized with IP or MAP respectively. We compared the immunogenicity of MAP with Lp.
Results:
The results showed that the immunogenicity of MAP which elicited high-titer antibody more than 1:40 960 was much stronger than that of LP (1:1 280).
Conclusions:
It suggests that the immunogenicity of peptide corresponding to HCV HVR1 was stronger by using MAP conformation, and this provide a basis for designing HCV molecular vaccine using MAP.