Related Experiment Videos
Mast cell MMP-9 production enhanced by bacterial lipopolysaccharide
A Tanaka1, Y Yamane, H Matsuda
1Department of Veterinary Clinic, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Abstract:
Although mast cells contribute to host protective immunity against bacterial infections, the exact mechanism of their recruitment at the affected site has been unclear. Recently, we have reported that both mouse and human mast cells are capable of producing matrix metalloproteinase (MMP)-9, a matrix-degrading enzyme necessary for leukocyte transmigration. Here, we demonstrated that bacterial lipopolysaccharide (LPS) enhanced MMP-9 production of mouse bone marrow derived-cultured mast cells. This action of LPS was partially suppressed by the pretreatment of cultured mast cells with a protein kinase C (PKC) inhibitor, indicating the possible involvement of PKC signaling pathways in the production of MMP-9 by LPS. Thus, these suggest the upregulation of mast cell MMP-9 by bacterial components, thereby resulting in their migration at the affected site.
Insights
Mast cells, crucial for fighting bacterial infections, migrate via matrix metalloproteinase (MMP)-9. Bacterial lipopolysaccharide (LPS) boosts mast cell MMP-9 production, a process involving protein kinase C (PKC) signaling.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Mast cells are vital immune cells involved in host defense against bacterial infections.
- The precise mechanisms governing mast cell recruitment to infection sites remain incompletely understood.
- Mast cells produce matrix metalloproteinase (MMP)-9, an enzyme facilitating leukocyte migration.
Purpose of the Study:
- To elucidate the role of bacterial components in mast cell migration.
- To investigate the regulation of MMP-9 production by mast cells in response to bacterial stimuli.
- To identify signaling pathways involved in LPS-induced MMP-9 production in mast cells.
Main Methods:
- Cultured mouse bone marrow-derived mast cells were utilized.
- Mast cells were stimulated with bacterial lipopolysaccharide (LPS).
- Protein kinase C (PKC) inhibitor was used to assess signaling pathway involvement.
Main Results:
- Bacterial LPS significantly enhanced MMP-9 production in mast cells.
- PKC inhibition partially abrogated the LPS-induced increase in MMP-9.
- These findings implicate PKC signaling in LPS-mediated MMP-9 upregulation.
Conclusions:
- Bacterial components, such as LPS, can upregulate mast cell MMP-9 production.
- This upregulation facilitates mast cell migration to sites of infection.
- PKC signaling pathways play a role in the response of mast cells to bacterial stimuli.