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Alpha(v)beta3 and alpha(v)beta5 integrin expression in glioma periphery
L Bello1, M Francolini, P Marthyn
1Institute of Neurosurgery, University of Milano, Ospedale Maggiore Policlinico, IRCCS, Italy. lbello@rics.bwh.harvard.edu
Neurosurgery
|August 16, 2001
Summary
Integrins alpha(v)beta3 and alpha(v)beta5 are expressed in glioma, with alpha(v)beta3 prominent in high-grade glioma periphery. These integrins play a role in glioma angiogenesis and cell migration.
Area of Science:
- Oncology
- Cell Biology
- Molecular Biology
Background:
- Gliomas are primary brain tumors with varying grades of malignancy.
- Integrins are cell surface receptors involved in cell adhesion, migration, and angiogenesis.
- Integrins alpha(v)beta3 and alpha(v)beta5 are implicated in tumor progression and angiogenesis.
Purpose of the Study:
- To analyze the expression of integrins alpha(v)beta3 and alpha(v)beta5 in glioma tissues.
- To investigate the role of these integrins in the periphery of high-grade gliomas.
Main Methods:
- Western blot, immunohistochemistry, and immunofluorescence were used to detect integrin expression.
- Monoclonal antibodies targeting functional integrin heterodimers were employed.
- Expression of integrin ligands and growth factors was also assessed.
Main Results:
- Both integrins alpha(v)beta3 and alpha(v)beta5 were expressed in glioma cells and vasculature, correlating with histological grade.
- Alpha(v)beta3 expression was prominent in astrocytic tumors and at the high-grade glioma periphery.
- Co-localization studies revealed alpha(v)beta3 with fibroblast growth factor 2 and matrix metalloproteinase 2 in tumor astrocytes and endothelial cells.
Conclusions:
- Integrins alpha(v)beta3 and alpha(v)beta5 are involved in glioma-associated angiogenesis.
- Integrin alpha(v)beta3 may play a significant role in neoangiogenesis and cell migration at the high-grade glioma periphery.