Related Experiment Video
Updated: Aug 13, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Sequence analysis shows that ribgrass mosaic virus Shanghai isolate (RMV-Sh) is closely related to Youcai mosaic
Abstract:
The complete nucleotide sequence of an isolate of Ribgrass mosaic virus (RMV-Sh) from Brassica chinensis (Qingcai) in Shanghai, China was determined. The genome consisted of 6301 nucleotides and its genomic organization was similar to those of other crucifer-tobamoviruses. Comparisons of the nucleotide and predicted amino acid sequences and phylogenetic analyses showed that RMV-Sh had very high homology (> 95% identical nucleotides and 97.7-99.6% identical amino acids) to a sequence of Youcai mosaic virus (YMV = Chinese rape mosaic or Oilseed rape mosaic virus), despite differences in host range or symptoms and this strongly suggests that these isolates should be regarded as belonging to the same species. Only coat protein sequences have been reported for other RMV isolates but it seems likely that the distinction between RMV and YoMV will be difficult to maintain.
Related Concept Videos
Viral Recombination
Viral Mutations
Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
Viruses of Archaea
Subviral Agents

