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[Determination of 4-O-methylpyridoxine (MPN) by HPLC with diode-array detector]
M Fujisawa1, M Nakajima, Y Hori
1Department of Analytical Chemistry, Niigata College of Pharmacy, Niigata.
Abstract:
We have developed a simple and rapid method for measuring 4-O-methylpyridoxine (MPN) in the serum of an acute poisoning patient by high-performance liquid chromatograph (HPLC) with a diode-array detector. MPN, a poisonous ingredient of ginkgo seeds was extracted using an Isolute C18 solid-phase extraction column. Equal volumes of the extract were injected into a C18 HPLC column using acetonitrile-10 mM phosphate buffer (pH 3) as the mobile phase. Excellent linearity was obtained over the range of 1-1000 ng at a wavelength of 290 nm, and its detection limit of approximately 0.5 ng was considered to be satisfactory. The mean recovery of MPN in the serum was 92.9 +/- 6.1%. The method was used successfully in a case of acute poisoning.
Insights
A new high-performance liquid chromatography method accurately measures 4-O-methylpyridoxine (MPN) in serum. This rapid technique aids in diagnosing acute poisoning from ginkgo seed ingestion.
Area of Science:
- Analytical Chemistry
- Toxicology
Context:
- Ginkgo seeds contain 4-O-methylpyridoxine (MPN), a toxic compound implicated in acute poisoning cases.
- Accurate and rapid quantification of MPN in biological samples is crucial for clinical diagnosis and management.
Purpose:
- To develop and validate a simple, rapid, and sensitive method for measuring MPN in human serum.
- To establish a reliable analytical technique for diagnosing acute MPN poisoning.
Summary:
- A high-performance liquid chromatography (HPLC) method with a diode-array detector was developed for MPN quantification.
- MPN was extracted using C18 solid-phase extraction and analyzed on a C18 column with an acetonitrile-phosphate buffer mobile phase.
- The method demonstrated excellent linearity (1-1000 ng), a low detection limit (~0.5 ng), and high mean recovery (92.9%).
Impact:
- This validated HPLC method provides a valuable tool for the timely and accurate diagnosis of acute ginkgo seed poisoning.
- The technique's simplicity and speed facilitate its application in clinical toxicology laboratories.
- Successful application in a real-world poisoning case underscores the method's clinical utility.