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Related Experiment Videos

Metabolic pattern of polymorphonuclear leucocytes induced by trypsin-digested microsomes.

K Takeshige, A Nakagawara, T Hatae

    Journal of Biochemistry
    |March 1, 1975
    PubMed
    Summary

    Trypsin-digested liver microsomes trigger cyanide-insensitive respiration and hexose monophosphate pathway acceleration in guinea pig immune cells, mimicking phagocytosis effects via cell membrane interaction.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Immunology

    Background:

    • Polymorphonuclear leukocytes (PMNs) play a crucial role in the immune response.
    • Phagocytosis in PMNs involves significant metabolic shifts, including increased respiration and hexose monophosphate (HMP) pathway activity.
    • The precise triggers for these metabolic changes are not fully understood.

    Purpose of the Study:

    • To investigate the effect of specific liver microsomal fractions on guinea pig PMN metabolism.
    • To determine if digested liver microsomes can induce metabolic changes similar to phagocytosis.

    Main Methods:

    • Guinea pig polymorphonuclear leukocytes were treated with trypsin-digested liver microsomes.
    • Respiration rates were measured, and sensitivity to mitochondrial and glycolytic inhibitors was assessed.

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  • Hexose monophosphate oxidative pathway activity was monitored.

    • Main Results:

    • Trypsin-digested liver microsomes induced cyanide-insensitive respiration in PMNs.
    • This respiration was sensitive to glycolytic inhibitors but not mitochondrial inhibitors.
    • Concomitant acceleration of the HMP oxidative pathway was observed.
    • Intact or differently treated microsomes (chymotrypsin, bacterial proteinase, ribonuclease, neuraminidase) did not elicit these responses.

    Conclusions:

    • Trypsin-digested liver microsomes can activate specific metabolic pathways in PMNs, independent of mitochondrial respiration.
    • These induced metabolic alterations resemble those occurring during phagocytosis.
    • The effect is likely mediated by interaction with the cell membrane, as microsomes were not internalized.