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Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
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No selection for CCR5 coreceptor usage during parenteral transmission of macrophagetropic syncytium-inducing human

F A Koning1, D Schols, H Schuitemaker

  • 1Department of Clinical Viro Immunology, CLB-Sanquin, and Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

Journal of Virology
|August 17, 2001
PubMed

Insights

Human immunodeficiency virus type 1 (HIV-1) transmitted variants showed selection for macrophage tropism. However, this tropism was not linked to CCR5 coreceptor usage, suggesting other factors are involved.

Area of Science:

  • Virology
  • Immunology
  • Infectious Diseases

Background:

  • Parenteral transmission of human immunodeficiency virus type 1 (HIV-1) can involve syncytium-inducing (SI) variants.
  • Macrophage tropism is a characteristic of certain HIV-1 variants, influencing disease progression.
  • Macrophage infection is typically mediated by the CCR5 coreceptor.

Purpose of the Study:

  • To investigate the tropism of transmitted HIV-1 SI variants in two cases of parenteral transmission.
  • To determine if CCR5 coreceptor usage is a selection factor for macrophagetropism in these transmitted variants.

Main Methods:

  • Analysis of HIV-1 variants selected following parenteral transmission.
  • Assessment of coreceptor usage (CCR5 and CXCR4) of transmitted SI variants.

Main Results:

  • Selection for macrophatropic variants was observed in both transmission cases.
  • No selection for SI variants utilizing CCR5 as a coreceptor was found.
  • Transmitted SI variants capable of infecting macrophages did not show a preference for CCR5 alongside CXCR4.

Conclusions:

  • Macrophage tropism of transmitted SI HIV-1 variants is not solely dependent on CCR5 coreceptor usage.
  • Factors beyond coreceptor usage likely contribute to the macrophatropism of these transmitted HIV-1 variants.

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