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Related Experiment Videos

Visualization of chromatin substructure: upsilon bodies.

A L Olins, R D Carlson, D E Olins

    The Journal of Cell Biology
    |March 1, 1975
    PubMed
    Summary

    Electron microscopy reveals spherical particles, termed upsilon bodies, in eukaryotic chromatin fibers. These structures, connected by filaments, suggest a particulate nature of native chromatin organization.

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    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biophysics

    Background:

    • Chromatin fibers are complex structures within eukaryotic nuclei.
    • Understanding chromatin's native configuration is crucial for comprehending nuclear processes.
    • Previous models proposed different arrangements for chromatin fibers.

    Purpose of the Study:

    • To investigate the ultrastructure of spread chromatin fibers.
    • To identify and characterize particulate components within chromatin.
    • To assess the relationship between observed structures and native chromatin.

    Main Methods:

    • Isolation of eucaryotic nuclei from rat thymus, rat liver, and chicken erythrocytes.
    • Spreading of chromatin fibers for electron microscopy.
    • Preparation of chromatin under dissociating solvent conditions.
    • Low-angle X-ray diffraction analysis of nuclear pellets.

    Main Results:

    • Linear arrays of spherical particles (upsilon bodies, ~70 A diameter) connected by thin filaments (~15 A wide) were observed.
    • These upsilon bodies were present in freshly isolated nuclei and prepared chromatin.
    • X-ray diffraction indicated minimal perturbation of native chromatin structure during preparation.

    Conclusions:

    • The observed upsilon bodies are likely integral components of native chromatin.
    • Chromatin may possess a particulate organization rather than a purely continuous structure.
    • These findings provide insights into the higher-order structure of chromatin.

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