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Updated: Aug 12, 2026
Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
Published on: July 6, 2014
Longitudinal analysis of histologic high-grade disease after negative cervical cytology according to endocervical
1Victorian Cervical Cytology Registry, Carlton South, Victoria, Australia. Heather.Mitchell@vccr.org
Background:
There is concern that Papanicolaou (Pap) smears without an endocervical component may be associated with the incomplete detection of abnormalities.
Methods:
Four cohorts of women with initial negative Pap smear reports and an additional Pap smear obtained within 36 months were established from a statewide database. The endocervical status of the entry smear and subsequent smears, respectively, among the cohort women was as follows: Cohort A: present and present; Cohort B: absent and present; Cohort C: present and absent; and Cohort D: absent and absent. The subsequent incidence of histologic high-grade disease was calculated for each cohort.
Results:
No significant differences were evident in the incidence of histologic high-grade disease between Cohorts A and B (standardized incidence ratio for Cohort B compared with Cohort A = 0.89; 95% confidence interval, 0.67-1.12). Cohorts C and D had significantly less high-grade disease compared with Cohorts A and B.
Conclusions:
The findings of the current study indicate that early repeat testing of women whose Pap smears are negative but lack an endocervical component is not justified because no higher rate of histologic high-grade abnormality was evident on longitudinal follow-up even when later smears included an endocervical component.
