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Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton.
S Uchiyama1, T Santa, N Okiyama
1Laboratory of Bio-Analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Biomedical Chromatography : BMC
|August 17, 2001
Summary
This review covers benzofurazan-based fluorogenic and fluorescent labeling reagents. It details their synthesis, reactivity, fluorescence, sensitivity, and applications for analyte detection.
Area of Science:
- Organic Chemistry
- Analytical Chemistry
- Biochemistry
Background:
- Benzofurazan (2,1,3-benzoxadiazole) derivatives are valuable fluorogenic and fluorescent labeling reagents.
- These reagents are crucial for sensitive detection and quantification of various analytes.
Purpose of the Study:
- To provide a comprehensive review of benzofurazan-based labeling reagents.
- To summarize their synthetic methodologies, chemical reactivity, and photophysical properties.
- To highlight their diverse applications in analytical chemistry.
Main Methods:
- Literature review of benzofurazan derivatives.
- Analysis of synthetic routes and reaction mechanisms.
- Evaluation of fluorescence characteristics (emission, excitation, quantum yield).
- Assessment of sensitivity and detection limits for various analytes.
Main Results:
- Detailed descriptions of reagents like NBD-F, DBD-F, ABD-F, SBD-F, NBD-H, DBD-H, NBD-PZ, DBD-PZ, DBD-COCl, and DBD-NCS.
- Comparison of their synthetic accessibility and stability.
- Characterization of their fluorescence spectra and quantum yields.
- Examples of their application in detecting biomolecules and other analytes.
Conclusions:
- Benzofurazan-based reagents offer versatile and sensitive labeling capabilities.
- The diverse functionalization of the benzofurazan core allows for tailored reagent design.
- These reagents are indispensable tools in modern analytical and biochemical research.