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The expression of gastric H+-K+-ATPase mRNA and protein in developing rat fundic gland

D H Yang1, S Tsuyama, F Murata

  • 1Department of Anatomy, Kagoshima University Faculty of Medicine, Japan.

The Histochemical Journal
|August 18, 2001
PubMed

Insights

Gastric acid production relies on the proton pump H+-K+-ATPase. This study shows that both H+-K+-ATPase mRNA and protein expression increase with age in developing rat stomachs, paralleling enzyme activity.

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Developmental Biology

Background:

  • The gastric proton pump, H+-K+-ATPase, is crucial for hydrochloric acid secretion by parietal cells.
  • Understanding the developmental regulation of H+-K+-ATPase is key to comprehending gastric acid physiology.

Purpose of the Study:

  • To investigate the association between H+-K+-ATPase mRNA and protein expression and the development of its activity in the rat fundic gland.
  • To track the ontogeny of H+-K+-ATPase expression and activity from late gestation through early postnatal life.

Main Methods:

  • H+-K+-ATPase activity was measured in rat fundic glands from gestational day 18.5 to postnatal 8 weeks.
  • In situ hybridization with a digoxigenin-labelled RNA probe and tyramide signal amplification detected H+-K+-ATPase mRNA expression.
  • Immunoblotting and immunohistochemistry using specific antibodies assessed H+-K+-ATPase alpha- and beta-subunit protein expression.

Main Results:

  • H+-K+-ATPase enzyme activity was detectable early in gland development (gestational day 19.5) and increased with age.
  • Both H+-K+-ATPase mRNA and protein expression were observed concurrently with enzyme activity.
  • A progressive increase in mRNA and protein expression was noted as rats matured.

Conclusions:

  • The expression of H+-K+-ATPase mRNA and protein in the developing rat fundic gland increases with age.
  • This developmental pattern of gene and protein expression parallels the maturation of H+-K+-ATPase enzyme activity.
  • These findings elucidate the molecular mechanisms underlying the development of gastric acid secretion capacity.

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