Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PLGA microsphere bioburden evaluation for radiosterilization dose selection.

A Gèze1, M C Venier-Julienne, J Cottin

  • 1UPRES EA 2169, Faculté de Pharmacie, Angers, France.

Journal of Microencapsulation
|August 18, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Publication bias in pharmacogenetics of adverse reaction to antiseizure drugs: An umbrella review and a meta-epidemiological study.

PloS one·2022
Same author

Prenatal constriction of ductus arteriosus following inadvertent maternal exposure to topical non-steroidal anti-inflammatory drug.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2021
Same author

Triamcinolone acetonide-loaded lipid nanocapsules for ophthalmic applications.

International journal of pharmaceutics·2019
Same author

Clomiphene citrate and neural tube defects: a meta-analysis of controlled observational studies.

BJOG : an international journal of obstetrics and gynaecology·2019
Same author

Neuromuscular blocking agents induced anaphylaxis: Results and trends of a French pharmacovigilance survey from 2000 to 2012.

Allergy·2018
Same author

Biodistribution and preliminary toxicity studies of nanoparticles made of Biotransesterified β-cyclodextrins and PEGylated phospholipids.

Materials science & engineering. C, Materials for biological applications·2018
Same journal

Rationally engineered essential oil-loaded nanocarriers for acne vulgaris: integrating multiscale molecular modeling, machine learning, and response surface optimization.

Journal of microencapsulation·2026
Same journal

Retinyl palmitate-loaded nanostructured lipid carriers prepared by the phase inversion temperature method: Physicochemical properties, <i>in vitro</i> skin permeation, and occlusion ability.

Journal of microencapsulation·2026
Same journal

Green synthesis of silver nanoparticles using <i>Swertia chirayita</i> and their antioxidant and anticancer potential.

Journal of microencapsulation·2026
Same journal

Management of coronary artery disease via simvastatin-loaded novasomes.

Journal of microencapsulation·2026
Same journal

Phyto-engineered CuO nanoparticles from gum <i>Eucalyptus camaldulensis</i>: a GC-MS, molecular docking, and bioactivity study.

Journal of microencapsulation·2026
Same journal

Development and optimization of gallic acid-enriched nanostructured lipid carriers for the amelioration of rheumatic inflammation: <i>in-vitro</i> and <i>in-vivo</i> study.

Journal of microencapsulation·2026
See all related articles

Determining the bioburden of PLGA microspheres led to a reduced gamma-irradiation sterilization dose. This lower dose better preserved polymer integrity and controlled drug release, minimizing radiation damage.

Area of Science:

  • Materials Science
  • Pharmaceutical Technology
  • Microbiology

Background:

  • Poly(lactic-co-glycolic) acid (PLGA) microspheres are widely used for drug delivery.
  • Sterilization of PLGA microspheres is crucial for safe pharmaceutical applications.
  • Gamma irradiation is a common sterilization method, but can affect polymer properties.

Purpose of the Study:

  • To determine the bioburden of PLGA microspheres.
  • To establish an appropriate gamma-irradiation dose for sterilization based on bioburden.
  • To evaluate the impact of a reduced irradiation dose on PLGA microspheres and drug release.

Main Methods:

  • PLGA microspheres were produced using the solvent emulsion/extraction process.
  • Bioburden analysis was performed on blank microspheres according to ISO specifications.

Related Experiment Videos

  • Microbial contamination was quantified as colony forming units (CFU).
  • Gamma irradiation doses were selected based on ISO standards and compared to European Pharmacopeia recommendations.

    • Main Results:

    • A mean bioburden of 36.04 CFU/110 mg of PLGA microspheres was determined.
    • A gamma-irradiation dose of 19.6 kGy was calculated as sufficient for a 10(-6) sterility level.
    • The reduced dose (19.6 kGy) resulted in less polymer molecular weight reduction compared to 25 kGy.
    • Controlled 5-fluorouracil (5-FU) drug release kinetics were observed with the lower irradiation dose.

    Conclusions:

    • The bioburden of PLGA microspheres allows for optimization of gamma-irradiation sterilization doses.
    • A reduced gamma-irradiation dose (19.6 kGy) effectively sterilizes PLGA microspheres while minimizing polymer degradation.
    • Optimized sterilization preserves PLGA microsphere integrity and enhances control over drug release profiles.