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Updated: Jul 28, 2026
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017
[Studies on cloning and expression of hepatitis B virus x gene]
1Central Laboratory, Shenyang Hospital for Infectious Diseases, Shenyang 110006, China.
Objective:
To express and purify the x gene of hepatitis B virus (HBV), and then the purity of the protein could specifically be analysed by the human antibodies against HBV x antigen (protein).
Methods:
HBV x gene was amplified from the complete genome by PCR, and cloned in the pGEX-2T fused expression vector. After transforming the plasmid into E.coli, the recombinant with HBx gene was obtained. The fusion protein was generated with E.coli fused expression system and purified with affinity chromatography.
Results:
The HBx protein could be applied to detecting specific antibody of hepatitis B virus patients.
Conclusions:
The purity of the HBx protein can specifically be recognized by the human antibodies against HBV x antigen.
