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[Annexin V technique for the study of liver damage]
1Department of Laboratory, Chongqing Medical University, Chongqing 400016, China.
Objective:
To evaluate Annexin V technique for measuring hepatic apoptosis and to investigate the protective function of Rg1 and Rb1 on acute liver injury.
Methods:
LPS-treated acute liver injury and the protective effect of Rg1 and Rb1 were assessed by Annexin V double staining and PI staining measurements. The activity of sPLA(2) was measured by [(3)H]-labelled oleic acid method.
Results:
Annexin V showed a higher sensitivity and specificity than PI staining. It was only the Annexin V assay that could discriminate normal cells, apoptosis cells, and necrotic cells. Rg1 and Rb1 could reduce the percentage of hepatic apoptotic and necrotic cells (P<0.01) and activity of sPLA2 (P<0.01).
Conclusions:
The Annexin V assay is an ideal method for measuring apoptosis presently. Rg1 and Rb1 have a definite protective effect on acute liver injury in rats.
