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Updated: Aug 18, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
[IFN-r/IL-4 expression in peripheral blood mononuclear cells of the patients with chronic severe hepatitis]
Objective:
To understand the expressions of interferon-r (INF-r) and interleukin-4 (IL-4) in peripheral blood mononuclear cells (PBMCs) of the patients with chronic severe hepatitis (CSH).
Methods:
PBMCs from the patients with CSH were separated routinely and stimulated by PMA/Ionomycin/Monensin. The production of IFN-r/IL-4 by CD4+ T cells in PBMC was determined by fluorescence activated call sorting (FACS) analysis and fluorescence-quantitative PCR assay for quantification of HBV DNA.
Results:
The percentage of IFN-gamma-producing T cells in CD4+ T cells was higher in patients with CSH (7.2%-26.3%) than in normal controls (2.2%-11.9%) (P<0.01). The percentage of Th1 cells increased significantly with the raise of hepatic inflammation activity. The percentage of IL-4-producing T cells in CD4+ T cells did not differ significantly between patients with CSH and normal controls. The quantification of HBV DNA decreased significantly with the increase of the percentage of IFN-gamma-producing T cells.
Conclusions:
Th1 cell is associated with hepatic inflammatory activity and IFN-gamma depresses HBV replication.
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