Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CpG methylation regulates the Igf2/H19 insulator.

C Holmgren1, C Kanduri, G Dell

  • 1Department of Development and Genetics, Uppsala University, Norbyvägen 18A, S-752 36, Uppsala, Sweden.

Current Biology : CB
|August 18, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[ON THE TRACES OF BRICS: INVOLVEMENT OF FOREIGN DONORS IN ADDRESSING THE CHALLENGES OF THE RUSSIAN BLOOD SERVICE].

Problemy sotsial'noi gigieny, zdravookhraneniia i istorii meditsiny·2025
Same author

Patient and provider preferences for long-acting TB preventive therapy.

IJTLD open·2025
Same author

Advanced therapy screening in inflammatory bowel disease and the impact of clinical nurse specialists: A retrospective analysis of electronic patient records.

Clinical medicine (London, England)·2025
Same author

Waste not, want not: measuring waste and potential clinical risk from limited gatekeeping.

Irish medical journal·2025
Same author

Dental Health Adjuncts and Care: Exploring Access Among Asylum Seekers and Refugees in London, United Kingdom.

JDR clinical and translational research·2024
Same author

Serum concentrations of legacy, alternative, and precursor per- and polyfluoroalkyl substances: a descriptive analysis of adult female participants in the MIREC-ENDO study.

Environmental health : a global access science source·2024

CpG methylation regulates the H19 insulator function, controlling communication between enhancers and promoters. This methylation mark dictates parent-specific chromatin conformations and represses maternal Igf2 expression.

Area of Science:

  • Genetics
  • Epigenetics
  • Genomic Imprinting

Background:

  • The H19 gene's 5' flank, differentially methylated, regulates enhancer-promoter communication and represses maternal Igf2 expression.
  • Paternally inherited Igf2 allele activation is linked to methylation-mediated inactivation of the H19 insulator during male germline development.

Purpose of the Study:

  • To investigate the role of CpG methylation in regulating the H19 insulator function.
  • To determine if methylation of the H19 imprinting control region (ICR) affects insulator activity and chromatin conformation.

Main Methods:

  • Insertion of a methylated H19 ICR fragment into a nonmethylated episomal H19 minigene.
  • Transfection into Hep3B cells and analysis of individual clones for genotype, methylation status, chromatin conformation, and insulator function.

Related Experiment Videos

Main Results:

  • Methylated status of the H19 ICR was maintained without spreading to the episomal vector.
  • Nuclease hypersensitive sites, typical of the maternal H19 ICR allele, were absent in the methylated ICR.
  • Insulator function was significantly attenuated in stably maintained episomes.

Conclusions:

  • CpG methylation of the H19 ICR is propagated independently of the episomal vector.
  • Methylation dictates parent of origin-specific chromatin conformations, involving CTCF.
  • Experimental evidence supports that CpG methylation regulates H19 insulator function.