Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human IgA activates the complement system via the mannan-binding lectin pathway.

A Roos1, L H Bouwman, D J van Gijlswijk-Janssen

  • 1Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands. A.Roos@LUMC.NL

Journal of Immunology (Baltimore, Md. : 1950)
|August 18, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The frequency of HLA-DQ7 in patients at risk of coeliac disease: A haplotype to be reckoned with for screening?

Human immunology·2024
Same author

Generation of two induced pluripotent stem cell lines (HIMRi006-A and HIMRi007-A) from Pompe patients with infantile and late disease onset.

Stem cell research·2024
Same author

Hippocampus, amygdala, and insula activation in response to romantic relationship dissolution stimuli: A case-case-control fMRI study on emerging adult students.

Journal of affective disorders·2024
Same author

Generation of two hiPSCs lines of two patients carrying truncating mutations in the dimerization domain of filamin C.

Stem cell research·2024
Same author

Prenatal alcohol exposure and white matter microstructural changes across the first 6-7 years of life: A longitudinal diffusion tensor imaging study of a South African birth cohort.

NeuroImage. Clinical·2024
Same author

Generation of two human iPSC lines (HIMRi002-A and HIMRi003-A) derived from Caveolinopathy patients with rippling muscle disease.

Stem cell research·2023

Mannan-binding lectin (MBL) binds to immunoglobulin A (IgA), activating the complement system. This interaction enhances innate immunity and may explain IgA nephropathy.

Area of Science:

  • Immunology
  • Innate Immunity
  • Complement System

Background:

  • The lectin pathway, initiated by mannan-binding lectin (MBL), is crucial for innate immunity.
  • MBL deficiency increases susceptibility to infections, particularly mucosal infections.
  • Immunoglobulin A (IgA) is a key mediator of mucosal immunity.

Purpose of the Study:

  • To investigate if IgA activates the complement system via the MBL-initiated lectin pathway.
  • To explore the mechanism of MBL-IgA interaction and its functional consequences.

Main Methods:

  • Dose-dependent MBL binding assays to immobilized IgA.
  • Calcium dependency and inhibition studies using mannose and anti-MBL antibodies.
  • Complement activation assays measuring C4 and C3 deposition.

Related Experiment Videos

  • Analysis of MBL-deficient and wild-type serum for IgA-induced complement activation.

    • Main Results:

    • MBL binds dose-dependently to polymeric IgA, involving its carbohydrate recognition domain.
    • MBL binding to IgA triggers complement activation (C4 and C3 deposition) at physiologically relevant concentrations.
    • Serum from MBL-deficient individuals shows reduced IgA-induced complement activation compared to wild-type.

    Conclusions:

    • MBL binding to IgA activates the complement system, suggesting a synergistic role in antimicrobial defense.
    • This interaction may enhance mucosal immunity and contribute to the pathogenesis of IgA nephropathy.