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Wistar strain rats as the model for IgE antibody experiments
T Hirano1, N Kawasaki, H Miyataka
1Department of Bio-organic Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
Biological & Pharmaceutical Bulletin
|August 21, 2001
Summary
Wistar rats produced the most Immunoglobulin E (IgE) antibody when exposed to a specific antigen and adjuvant. This finding suggests Wistar rats are a suitable model for studying allergic diseases.
Area of Science:
- Immunology
- Allergy Research
- Animal Models
Background:
- Plasma Immunoglobulin E (IgE) antibody levels are critical indicators in allergic responses.
- Understanding IgE production dynamics across different rat strains is essential for allergy research.
Purpose of the Study:
- To investigate and compare plasma IgE antibody formation over time in Sprague-Dawley (SD), Donryu, and Wistar rat strains.
- To determine the optimal rat strain for modeling allergic diseases based on IgE production.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was employed to quantify plasma IgE antibody levels.
- Antigenic stimulation involved injecting 2,4-dinitrophenylated ascaris extract (DNP-As) with killed Bordetella pertussis adjuvant into rat paws.
Main Results:
- SD and Donryu rat strains showed low IgE levels on day 10, with increases by day 20.
- Wistar strain rats exhibited peak IgE antibody levels on day 10 (130 ng/ml in males, 200 ng/ml in females).
Conclusions:
- Wistar strain rats demonstrate significantly higher IgE antibody production compared to SD and Donryu strains when stimulated with DNP-As.
- Wistar rats are proposed as a valuable animal model for investigating allergic diseases due to their robust IgE response.