Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Limbal fibroblast conditioned media: a non-invasive treatment for limbal stem cell deficiency.

Molecular vision·2011

Same author

Down-regulation of Notch signaling during corneal epithelial proliferation.

Molecular vision·2008

Same author

The tumor suppressor effect of the glucocorticoid receptor in skin is mediated via its effect on follicular epithelial stem cells.

Oncogene·2006

Same author

Plasminogen activator inhibitor type 2 (PAI-2) is present in normal human conjunctiva.

Journal of cellular physiology·2005

Same author

Reconstruction of the ocular surface by transplantation of a serum free cultivated conjunctival tissue equivalent.

Annals of the Academy of Medicine, Singapore·2005

Same author

Epithelial stem cells: the eye provides a vision.

Eye (London, England)·2003

Search research articles

Related Experiment Video

Updated: Jan 6, 2026

Isolation of Preadipocytes from Broiler Chick Embryos

Isolation of Preadipocytes from Broiler Chick Embryos

Published on: August 4, 2022

Lipid synthesis in chick epidermis.

The Journal of Investigative Dermatology

|July 1, 1975

Summary

This summary is machine-generated.

Newborn chick epidermis synthesizes triglycerides, primarily in basal and spinous cells. These lipids form droplets and are incorporated into horny cells during keratinization, revealing lipid synthesis pathways.

More Related Videos

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Published on: May 15, 2019

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Published on: March 10, 2016

Related Experiment Videos

Last Updated: Jan 6, 2026

Isolation of Preadipocytes from Broiler Chick Embryos

Isolation of Preadipocytes from Broiler Chick Embryos

Published on: August 4, 2022

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Published on: May 15, 2019

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Published on: March 10, 2016

Area of Science:

Dermatology
Biochemistry
Cell Biology

Background:

The epidermis forms a protective barrier.
Lipid synthesis is crucial for epidermal function and barrier integrity.
Understanding lipid metabolism in epidermal development is key.

Purpose of the Study:

To investigate lipid synthesis in newborn chick epidermis.
To identify the specific lipids synthesized and their cellular localization.
To elucidate the role of cellular organelles in lipid metabolism.

Main Methods:

Electron microscopic autoradiography was employed.
Tritiated palmitate was used as a radioactive precursor.
Cellular distribution and localization of synthesized lipids were analyzed over time.

Main Results:

Triglycerides were identified as the primary labeled lipid product.
Lipid incorporation was highest in basal and spinous epidermal cells.
Synthesized triglycerides were mainly aggregated into lipid droplets within the endoplasmic reticulum.
Lipids were retained in horny cells, indicating their contribution to keratinization.

Conclusions:

Epidermal cells synthesize triglycerides, with active synthesis occurring in basal and spinous layers.
The endoplasmic reticulum plays a role in forming lipid droplets.
Lipids are an integral component of the horny layer, contributing to the epidermal barrier function.