[Native magnetic resonance tomography imaging of the Morris hepatoma (MH-7777A) in the rat]
Related Experiment Video
Updated: Jul 27, 2026
Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures
Published on: October 20, 2009
G Krupski1, D Ameis, G Cataldegirmen
1Klinik und Poliklinik für Radiologie, Universitätsklinikum Hamburg-Eppendorf.
Unlabelled:
Goal of the study presented was to establish an oncological animal model for implantable and differentiated hepatoma in the rat and to evaluate imaging of the tumor induced using MRI.
Material And Methods:
20 male buffalo rats underwent tumor cell implantation of 150,000 MH7777-A cells via laparotomy. After 12 days MRI was performed T1w SE, T2w TSE fs, TIRM) for tumor detection and measurement of size. Immediately there after all rats were killed and macroscopic and histological examination performed. Pathological findings were correlated with MRI.
Results:
In 5 out of 20 animals no tumor was found. Mean size of the hepatomas in 15 animals was 3.1 mm (+/- 1.7 mm). On MRI 14 out of 15 tumors were detectable. Mean diameter in MRI was 2.7 mm (+/- 1.5 mm; 1-5 mm). T2w TSE was superior for tumor detection compared with the TIRM, while the best anatomic depiction was offered by T1w SE.
Conclusions:
The MH7777-A tumor model presents with a rate of 75% even with the small tumor cell amount of 150,000 cells. With MRI using T2 weighted sequences with fat saturation a detection of tumors with diameters of 1 mm is reliably possible in 93% of the cases.
