Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inflammatory-cell subpopulations in keloid scars.

D E Boyce1, J Ciampolini, F Ruge

  • 1Department of Plastic Surgery, Diana, Princess of Wales Children's Hospital, Birmingham, UK.

British Journal of Plastic Surgery
|August 22, 2001
PubMed
Summary

Keloid scars show increased numbers of macrophages and lymphocytes compared to normal skin. An altered CD4:CD8 ratio in keloid tissue suggests immune cell imbalance contributes to scar formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wound healing: potential therapeutic options.

The British journal of dermatology·2021
Same author

Development and validation of a gene expression test to identify hard-to-heal chronic venous leg ulcers.

The British journal of surgery·2019
Same author

Managing Wound Infection.

Journal of wound care·2016
Same author

The use of antiseptics in wound care: Critique II.

Journal of wound care·2016
Same author

Wound infection: Managing wound infection.

Journal of wound care·2016
Same author

Editorial.

Journal of wound care·2016

Area of Science:

  • Dermatology
  • Immunology
  • Pathology

Background:

  • Keloid scarring is a complex dermatological condition.
  • The role of inflammatory cells in keloid pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the contribution of lymphocytes and macrophages to keloid scarring.
  • To morphologically characterize inflammatory cell subpopulations in keloid scars versus normal skin.

Main Methods:

  • Punch biopsies from keloid scars and normal skin were obtained.
  • Tissue sections were stained with anti-inflammatory cell monoclonal antibodies.
  • Macrophage and lymphocyte subpopulations were quantified and compared.

Main Results:

Related Experiment Videos

  • Significantly higher numbers of macrophages and lymphocytes were observed in keloid dermis.
  • No significant increase in lymphocyte activation markers (CD25, CD27) was found.
  • A significantly higher CD4(+):CD8(+) (T helper: T suppressor) ratio was detected in keloid tissue.

    • Conclusions:

    • Increased macrophage and lymphocyte infiltration contributes to keloid scarring.
    • An imbalance in T helper and T suppressor cell populations may play a role in keloid formation.