Related Experiment Video
Updated: Jul 28, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
Published on: June 20, 2018
A polymorphic marker for the human cathepsin B gene
J R MacKenzie1, S L Mason, J G Hickford
1Department of Biochemistry and Molecular Biology, the John Curtin School of Medical Research, Australian National University, Canberra, Australia. jason.mackenzie@anu.edu.au
Abstract:
Human cathepsin B (CTSB) is a proteolytic enzyme implicated in tumor invasion and metastasis. We describe a PCR-based polymorphic marker for this gene comprising two amplimers differing in length by 19 consecutive nucleotides in intron 7, near the exon 8 splice acceptor site, identifying two gene alleles (A and B). Allele frequencies were 0.614 for A and 0.386 for the B allele, with an observed heterozygosity of 0.457 in a cohort of 70 non-related Australian blood donors. One additional nucleotide difference was also revealed through sequencing. The human CTSB gene is located on chromosome 8 and the alleles described here can potentially be used as markers in linkage and association studies of cancers and other diseases.

