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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
09:46

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

Published on: February 25, 2010

Vector for pop-in/pop-out gene replacement in Pichia pastoris

J Soderholm1, B J Bevis, B S Glick

  • 1University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.

Biotechniques
|August 23, 2001
PubMed
Summary

Researchers developed a new counterselectable marker, T-urf13, for Pichia pastoris gene replacement. This maize-derived gene offers a faster alternative to URA3, improving yeast strain usability.

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