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Related Experiment Videos

CD3 polymorphism in cynomolgus monkeys (Macaca fascicularis).

A Uda1, K Tanabayashi, R Mukai

  • 1Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Ibaraki, Japan.

Journal of Medical Primatology
|August 23, 2001
PubMed
Summary

Researchers found variations in the CD3epsilon molecule of cynomolgus monkeys, impacting lymphocyte reactivity. This polymorphism at the epitope level, recognized by the FN18 antibody, explains differences in immune responses.

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Identification of an amino acid responsible for the CD3 polymorphism in cynomolgus monkeys (Macaca fascicularis).

Journal of medical primatology·2003

Area of Science:

  • Immunology
  • Molecular Biology
  • Primate Research

Background:

  • The CD3 complex is crucial for T cell receptor signaling.
  • Monoclonal antibodies are vital tools for characterizing cell surface molecules.
  • Understanding immune system variations in non-human primates is essential for translational research.

Purpose of the Study:

  • To investigate the basis of differential lymphocyte reactivity to the FN18 monoclonal antibody in cynomolgus monkeys.
  • To identify molecular differences in the CD3 complex associated with non-reactivity.

Main Methods:

  • Lymphocyte reactivity assays using the FN18 monoclonal antibody.
  • Nucleotide sequencing of CD3delta, CD3gamma, and CD3epsilon chains.
  • Comparative amino acid sequence analysis.

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Main Results:

  • 12.2% of cynomolgus monkeys (24/196) showed non-reactive lymphocytes with the FN18 antibody.
  • T cells from non-reactive monkeys responded normally to mitogenic stimulation.
  • Two amino acid differences were identified in the CD3epsilon chain of non-reactive monkeys compared to reactive ones.

Conclusions:

  • The CD3epsilon molecule in cynomolgus monkeys exhibits epitope-level polymorphism.
  • This polymorphism, recognized by the FN18 antibody, accounts for observed variations in lymphocyte reactivity.
  • The findings highlight the importance of considering molecular variations in primate immunology studies.