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Related Experiment Videos

Assay for acetazolamide in plasma.

W F Bayne, G Rogers, N Crisologo

    Journal of Pharmaceutical Sciences
    |March 1, 1975
    PubMed
    Summary

    A new method accurately measures acetazolamide in plasma, crucial for understanding drug effectiveness. This sensitive assay aids in pharmacokinetic studies of acetazolamide formulations.

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    Area of Science:

    • Pharmacology
    • Analytical Chemistry

    Background:

    • Acetazolamide is a carbonic anhydrase inhibitor used to treat glaucoma and epilepsy.
    • Accurate quantification of acetazolamide in biological matrices is essential for pharmacokinetic and therapeutic drug monitoring.

    Purpose of the Study:

    • To develop and validate a sensitive analytical method for determining acetazolamide concentrations in human plasma.
    • To enable the study of plasma level profiles of various acetazolamide dosage forms.

    Main Methods:

    • Plasma samples were extracted using ethyl acetate, followed by lipid removal with methylene chloride.
    • Acetazolamide and its propionyl analog (internal standard) were analyzed using high-performance liquid chromatography (HPLC) on an octadecyl trichlorosilane bonded phase.
    • The method achieved a sensitivity of 25 ng/ml.

    Main Results:

    • The developed HPLC method demonstrated good sensitivity and specificity for acetazolamide analysis in plasma.
    • The extraction and chromatographic conditions were optimized for efficient separation and quantification.
    • The method is suitable for analyzing plasma samples in pharmacokinetic studies.

    Conclusions:

    • A reliable and sensitive HPLC method for acetazolamide quantification in plasma has been established.
    • This method facilitates the comparative study of different acetazolamide dosage forms.
    • The assay provides a valuable tool for pharmacokinetic research and clinical applications of acetazolamide.

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