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Development and evaluation of method for simultaneous determination of phenobarbital and diphenylhydantoin in plasma

S H Atwell, V A Green, W G Haney

Journal of Pharmaceutical Sciences

|May 1, 1975

Abstract:

A procedure for the simultaneous analysis of diphenylhydantoin and phenobarbital in plasma by high-pressure liquid chromatography was developed and evaluated. Separation is effected on a porous particle silicic acid column with chloroform dioxane-isopropanol-acetic acid (310:9.7:1.0:0.1 by volume) and is monitored at 254 nm. Results of the procedure were compared with results of a GLC assay.

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