Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evaluation of polyplexes as gene transfer agents.

C L Gebhart1, A V Kabanov

  • 1Department of Pharmaceutical Sciences, College of Pharmacy, 986025 Nebraska Medical Center, Omaha, NE 68198-6025, USA.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|August 23, 2001
PubMed
Summary

Non-viral polyplexes, complexes of DNA and polycations, show high gene transfection efficiency with low toxicity. Several polycation systems, including linear polyethyleneimine and dendrimers, outperform traditional lipid-based reagents, offering a promising alternative for gene delivery applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multilayer polyion complex nanoformulations of superoxide dismutase 1 for acute spinal cord injury.

Journal of controlled release : official journal of the Controlled Release Society·2017
Same author

In silico design of high-affinity ligands for the immobilization of inulinase.

Computers in biology and medicine·2016
Same author

Parvovirus associated fulminant hepatic failure and aplastic anemia treated successfully with liver and bone marrow transplantation. A report of two cases.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2014
Same author

Targeted delivery of cisplatin by сonnexin 43 vector nanogels to the focus of experimental glioma C6.

Bulletin of experimental biology and medicine·2014
Same author

Visualization of experimental glioma C6 by MRI with magnetic nanoparticles conjugated with monoclonal antibodies to vascular endothelial growth factor.

Bulletin of experimental biology and medicine·2013
Same author

[Production of timolol containing calcium-phosphate nanoparticles and evaluation of their effect on intraocular pressure in experiment].

Vestnik oftalmologii·2012

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Materials Science

Background:

  • Gene transfection is crucial for molecular biology research and therapeutic applications.
  • Non-viral gene delivery methods are sought to overcome the limitations of viral vectors, such as safety and immunogenicity.
  • Polycation-DNA complexes, or polyplexes, represent a promising non-viral gene delivery strategy.

Purpose of the Study:

  • To evaluate the efficacy, toxicity, and cell-type dependence of various non-viral polycation-based transfection systems.
  • To compare the performance of polyplexes with established lipid-based transfection reagents.
  • To identify optimal polyplex formulations for efficient and safe gene delivery.

Main Methods:

  • Synthesis and characterization of diverse polycations, including branched and linear polyethyleneimines, pyridinium bromide polymers, dendrimers (Superfect, Astramol), and a Pluronic-polyethyleneimine conjugate (P123-g-PEI(2K)).

Related Experiment Videos

  • In vitro transfection assays using a panel of cell lines to assess gene delivery efficiency.
  • Cytotoxicity assays to evaluate the safety profile of the tested polyplex systems.
  • Comparative analysis against commercial lipid-based transfection reagents (Lipofectin, LipofectAMINE, CeLLFECTIN, DMRIE-C).

    • Main Results:

    • Linear polyethyleneimine (ExGen 500), Superfect, branched polyethyleneimine (25 kDa), and P123-g-PEI(2K) demonstrated the highest transfection activity.
    • These leading polyplex systems exhibited significantly lower cytotoxicity compared to lipid-based transfection reagents.
    • The identified polyplex systems showed comparable or superior transfection efficiency to lipid reagents, without serum dependence.
    • Polycation structure significantly influenced transfection efficacy and cytotoxicity.

    Conclusions:

    • Structurally diverse polyplex systems hold significant potential as effective and low-toxicity non-viral gene transfection reagents.
    • Specific polycations, including linear PEI and dendrimers, offer advantages over traditional lipid-based methods.
    • Further development of polyplex formulations could lead to improved gene delivery strategies for research and therapeutic purposes.