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Automated constant-current coulometric assay system for ascorbic acid and sodium ascorbate.

S A Moros, C M Hamilton, J E Heveran

    Journal of Pharmaceutical Sciences
    |July 1, 1975
    PubMed
    Summary
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    An automated coulometric system accurately and precisely assays ascorbic acid and sodium ascorbate. This system analyzes 25 samples unattended for 2.5 hours with +/- 0.3% accuracy.

    Area of Science:

    • Analytical Chemistry
    • Electrochemistry

    Background:

    • Ascorbic acid and sodium ascorbate are vital compounds with significant analytical requirements.
    • Automated analytical systems offer enhanced efficiency and reliability in chemical assays.

    Purpose of the Study:

    • To evaluate the performance of an automated constant-current coulometric system for quantifying ascorbic acid and sodium ascorbate.
    • To determine the accuracy and precision of the automated system under optimized electrochemical conditions.

    Main Methods:

    • Utilized a constant-current coulometric titration system for automated sample analysis.
    • Optimized electrochemical parameters to ascertain optimal assay conditions.
    • Evaluated system performance based on accuracy and precision metrics.

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    Main Results:

    • The automated system demonstrated capability for unattended analysis of 25 samples over 2.5 hours.
    • Optimized conditions yielded high accuracy and precision, with results within +/- 0.3% (95% confidence).

    Conclusions:

    • The described automated coulometric system provides a reliable and efficient method for the assay of ascorbic acid and sodium ascorbate.
    • The system's high accuracy and precision make it suitable for routine quality control and research applications.