Related Experiment Videos
Antimicrobial susceptibility of potentially pathogenic halophilic vibrios isolated from seafood
D Ottaviani1, I Bacchiocchi, L Masini
1Institute of Experimental Veterinary Medicine Umbria-Marche, Cupa di Posatora, 3, 06100 Ancona, Italy. d.ottaviani@pg.izs.it
Abstract:
Susceptibility patterns to 27 antimicrobial agents and beta-lactamase production were investigated in potentially pathogenic halophilic vibrios from seafood. The effect of salinity on the response to the drugs in vitro was also studied. All isolates were uniformly sensitive to choramphenicol, imipenem, meropenem but resistant to lincomycin. All were highly sensitive to oxolinic acid, trimethoprim-sulphamethoxazole, doxycycline, flumequine, cefotaxime, nalidixic acid and ciprofloxacin. Some strains of V. harveyi, V. alginolyticus and V. parahaemolyticus apparently had mechanisms of resistance to several beta-lactam antibiotics other than by the production of beta-lactamases. Sixty-nine strains produced penicillinase but a low correlation between beta-lactamase activity and resistance to beta-lactam antibiotics was noted. The salt concentration affected the in vitro susceptibility of halophilic vibrios and the effect of salinity depended on both the individual strains and the antimicrobial tested.
Insights
This study examined antimicrobial susceptibility in seafood-derived halophilic vibrios. While uniformly sensitive to certain drugs and resistant to others, salinity significantly impacted their drug response.
Area of Science:
- Microbiology
- Food Safety
- Antimicrobial Resistance
Background:
- Halophilic vibrios are potential pathogens found in seafood.
- Understanding their antimicrobial susceptibility is crucial for public health and food safety.
Purpose of the Study:
- To investigate the antimicrobial susceptibility patterns of pathogenic halophilic vibrios from seafood.
- To determine the influence of salinity on the in vitro drug response of these bacteria.
Main Methods:
- Testing susceptibility to 27 antimicrobial agents.
- Assessing beta-lactamase production.
- Evaluating drug response under varying salinity conditions.
Main Results:
- All isolates were sensitive to chloramphenicol, imipenem, meropenem, and resistant to lincomycin.
- High sensitivity was observed for oxolinic acid, trimethoprim-sulphamethoxazole, doxycycline, flumequine, cefotaxime, nalidixic acid, and ciprofloxacin.
- Salinity affected in vitro susceptibility, with strain- and drug-specific responses.
Conclusions:
- Halophilic vibrios exhibit varied antimicrobial resistance profiles.
- Mechanisms of beta-lactam resistance may exist beyond beta-lactamase production.
- Salinity is a critical factor influencing the efficacy of antimicrobial agents against these marine bacteria.