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Related Experiment Videos

Expression and function of the HSD-3.8 gene encoding a testis-specific protein.

W Lin1, X Zhou, M Zhang

  • 1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan 3-Tiao, Beijing 100005, People's Republic of China.

Molecular Human Reproduction
|August 23, 2001
PubMed
Summary

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This study identifies HSD-3.8, a human sperm protein, as a GTP binding protein. This discovery suggests a potential new target for male fertility regulation and contraception development.

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The HSD-3.8 cDNA encodes a human sperm component.
  • Understanding its function is crucial for reproductive health research.

Purpose of the Study:

  • To characterize the HSD-3.8 protein.
  • To investigate its role in human spermatozoa and germ cells.
  • To assess its potential as an immunogen for fertility control.

Main Methods:

  • Nucleotide sequencing of HSD-3.8 cDNA.
  • Construction and expression of recombinant fusion proteins.
  • Immunostaining of human spermatozoa and testis tissue.
  • Fertility assessment in immunized female rats.
  • Biochemical assays for GTP binding and phosphorylation.

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Main Results:

  • HSD-3.8 is a 926-amino acid polypeptide with a tetratricopeptide repeat motif.
  • Recombinant HSD-3.8 localized to the postacrosomal zone of spermatozoa and germ cells.
  • Immunization with HSD-3.8 reduced fertility in female rats.
  • HSD-3.8 is a GTP binding protein with GTPase activity, regulated by phosphorylation.

Conclusions:

  • HSD-3.8 is a novel GTP binding protein found in human sperm.
  • Its presence in germ cells and effect on fertility suggest a role in reproduction.
  • HSD-3.8 represents a potential target for male contraceptive strategies.