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Predicted amino acid sequences for 100 JCV strains
C L Cubitt1, X Cui, H T Agostini
1Neurotoxicology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
Journal of Neurovirology
|August 23, 2001
Summary
Amino acid variations in the John Cunningham virus (JCV) can affect its neurovirulence potential. This study analyzed 100 JCV genomes to identify type-specific amino acid variants across its 8 genotypes.
Area of Science:
- Virology
- Genetics
- Neuroscience
Background:
- John Cunningham virus (JCV) exhibits genetic diversity among its 8 genotypes.
- While DNA sequence variation is mainly in noncoding regions, amino acid sequence differences are suspected to influence JCV's neurovirulence.
Purpose of the Study:
- To investigate the relationship between JCV genotype and amino acid sequence variation.
- To identify specific amino acid variants associated with different JCV genotypes and their potential impact on neurovirulence.
Main Methods:
- Translation of amino acid sequences from 100 JCV genomes.
- Grouping of translated sequences into distinct genotype families.
- Determination of subtype consensus sequences and identification of type-specific amino acid variants.
Main Results:
- Identification of specific amino acid sequence variants unique to different JCV genotypes.
- Characterization of the diversity in amino acid sequences across the 8 JCV genotypes.
Conclusions:
- Amino acid sequence variation is a key feature differentiating JCV genotypes.
- These identified variants provide a basis for further research into the mechanisms of JCV neurovirulence.