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Separation of low molecular weight proteins with SDS-PAGE using taurine as a new trailing ion
P Ehlermann1, U Redweik, N Blau
1Department of Medicine II, Medical University of Lübeck, Germany.
General Physiology and Biophysics
|August 25, 2001
Abstract:
Taurine as an alternative trailing ion for tricine yields an identical resolution but reduces the running time by 15% and the power consumption by 15% compared to tricine. Therefore cooling of gels is more effective in SDS PAGE with taurine and artefacts due to oxidation of proteins can thus be reduced.