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Continuous superporous agarose beds in radial flow columns
1Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Sweden. per-erik.gustavsson@tbiokem.lth.se
Journal of Chromatography. A
|August 25, 2001
Summary
Superporous agarose beds offer a novel chromatography support. These continuous gel beds with tunable pores show promise in affinity chromatography and biocatalysis applications.
Area of Science:
- Biochemistry
- Materials Science
- Chemical Engineering
Background:
- Continuous superporous agarose beds are a novel support material.
- These beds feature homogeneously transected, flow-carrying pores (10-100 microm).
Purpose of the Study:
- To prepare and characterize large-diameter (60 mm) superporous agarose beds.
- To evaluate their utility in chromatography, biocatalysis, and electrophoresis.
Main Methods:
- Preparation of large-diameter superporous agarose beds.
- Investigation of chromatographic properties using size-exclusion chromatography.
- Application in affinity chromatography (Cibacron Blue 3GA derivatization) and biotransformation (immobilized beta-galactosidase).
Main Results:
- Successfully prepared large-diameter superporous agarose beds.
- Demonstrated effectiveness in size-exclusion chromatography.
- Achieved purification of lactate dehydrogenase and production of lactose-free milk.
Conclusions:
- Superporous agarose beds are versatile for chromatography and biocatalysis.
- The tunable pore size and continuous nature offer advantages for various applications.