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Updated: Aug 14, 2026
11:56
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Vitamin C is reduced in human milk after storage
I H Buss1, F McGill, B A Darlow
1Department of Pathology, University of Otago, Christchurch School of Medicine, New Zealand. hendrikje.buss@chmeds.ac.nz
Acta Paediatrica (Oslo, Norway : 1992)
|August 25, 2001
Summary
Cold storage significantly reduces bioavailable vitamin C in human milk. Refrigeration for 24 hours or freezing for 1 month is recommended to preserve vitamin C levels.
Area of Science:
- Nutritional Science
- Biochemistry
Background:
- Human milk is a vital source of nutrients for infants.
- Bioavailable vitamin C (ascorbic acid) is crucial for infant development and immunity.
- Understanding the impact of storage on vitamin C is essential for infant nutrition.
Purpose of the Study:
- To determine the effect of cold storage conditions on bioavailable vitamin C levels in human milk.
- To quantify vitamin C degradation during refrigeration and freezing.
Main Methods:
- 11 human milk samples were analyzed.
- Samples were stored at 24 hours in a refrigerator.
- Samples were stored for up to 2 months in a freezer.
Main Results:
- Total vitamin C decreased by approximately one-third after 24 hours refrigeration.
- Freezing for 1 month resulted in an average decrease of one-third, with significant individual variability (6-76%).
- Freezing for 2 months led to an average decrease of two-thirds (7-100%).
Conclusions:
- Current human milk storage guidelines may lead to substantial vitamin C loss.
- Recommendations include storing milk in the refrigerator for under 24 hours or freezing for under 1 month.
- Vitamin C supplementation for infants may be a viable alternative to ensure adequate intake.
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