Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in the Ca2+-activated K+ current of pulmonary arterial smooth muscle in pulmonary hypertension rats.

S Muraki1, N Tohse, S Seki

  • 1Department of Physiology, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.

Naunyn-Schmiedeberg'S Archives of Pharmacology
|August 25, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Copy number elevation of 22q11.2 genes arrests the developmental maturation of working memory capacity and adult hippocampal neurogenesis.

Molecular psychiatry·2017
Same author

Pungent Alkamides from Spilanthes acmella L. var. oleracea Clarke.

Bioscience, biotechnology, and biochemistry·2016
Same author

[JSNP Excellent Presentation Award for CINP2014].

Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology·2015
Same author

Block of postjunctional muscle-type acetylcholine receptors in vivo causes train-of-four fade in mice.

British journal of anaesthesia·2015
Same author

Bio-inspired low frictional surfaces having micro-dimple arrays prepared with honeycomb patterned porous films as wet etching masks.

Langmuir : the ACS journal of surfaces and colloids·2014
Same author

Robust platforms for creating organic-inorganic nanocomposite microspheres: decorating polymer microspheres containing mussel-inspired adhesion layers with inorganic nanoparticles.

Chemical communications (Cambridge, England)·2014

Pulmonary hypertension in rats shows reduced Ca2+-activated K+ channel activity in pulmonary artery smooth muscle cells. This decrease in outward currents may contribute to vascular hyperreactivity and increased mortality in pulmonary hypertension.

Area of Science:

  • Cardiovascular Physiology
  • Smooth Muscle Cell Biology
  • Pulmonary Vascular Disease

Background:

  • Pulmonary hypertension is characterized by elevated vascular tone and hyperreactivity, contributing to mortality.
  • Understanding the cellular mechanisms underlying pulmonary hypertension is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the characteristics of membrane currents in pulmonary artery smooth muscle cells from rats with monocrotaline-induced pulmonary hypertension.
  • To determine the role of Ca2+-activated K+ channels in the pathophysiology of pulmonary hypertension.

Main Methods:

  • Monocrotaline-induced pulmonary hypertension model in male Wistar rats.
  • Whole-cell patch clamp technique to record membrane currents in isolated pulmonary artery smooth muscle cells.

Related Experiment Videos

  • Assessment of outward currents and the effects of Ca2+-activated K+ channel blockers.

    • Main Results:

    • Pulmonary artery smooth muscle cells from monocrotaline-injected rats exhibited smaller outward current densities compared to controls when intracellular calcium was buffered with 0.1 mM EGTA.
    • Increasing EGTA concentration to 10 mM equalized outward current densities, suggesting a role for calcium-activated currents.
    • Ca2+-activated K+ channel blockers and nisoldipine were less effective in monocrotaline-injected rats.
    • Depolarization induced by 4-aminopyridine was greater in monocrotaline-injected rats, indicating reduced Ca2+-activated K+ channel activity.

    Conclusions:

    • Ca2+-activated K+ channel activity is decreased in pulmonary hypertension.
    • Reduced activity of these channels may contribute to the vascular hyperreactivity observed in pulmonary hypertension.
    • These findings offer insights into the cellular basis of pulmonary hypertension and potential therapeutic targets.