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Hypertensive vascular lesions and renin or lysosomal enzymes in rats
Abstract:
In rats treated with DOCA plus high salt or with high salt alone, hypertensive rats with renal vascular lesions showed an incomplete suppression of KRA. Cathepsin activity of rat kidney was higher under high salt loading than in the control. Beta-glucuronidase activity was greatest in rats with renal vascular lesions and smallest in rats fed on normal chow. RNase and DNase activities were greater in rats with renal vascular lesions than in rats without renal vascular lesions under high salt loading. 2) In rats of both sexes SHR showed greater KRA and cathepsin activities than WK rat under high salt loading. In female rats DNase, RNase and beta-GPase activities were greater in SHR than in WK rat under high salt loading. 3) KRA was higher in SHRSP aged 10 months than in SHRSR, though KRA of SHR was smaller than KRA of WK rat. Cathepsin activity was greater in SHRSP than in SHRSR. DNase and beta-NAGA activities were greater in SHR than in WK rat. 4) In 7 weeks of age SHRSR showed more PRC than SHRSP. At the age of 10 months SHRSP showed higher PRC than WK rat. The roles renin and lysosomal enzymes in hypertensive renal vascular lesions were discussed to some extent.
Insights
Hypertension in rats is linked to altered kidney enzyme activities, including renin, cathepsin, and lysosomal enzymes. These changes correlate with renal vascular lesions, suggesting their role in hypertensive disease progression.
Area of Science:
- Biochemistry
- Nephrology
- Pathology
Background:
- Hypertension is associated with renal vascular lesions.
- The role of specific kidney enzymes in hypertension requires further elucidation.
Purpose of the Study:
- To investigate the activities of various kidney enzymes in different rat models of hypertension.
- To correlate enzyme activities with the presence and severity of renal vascular lesions.
Main Methods:
- Assessing renin, cathepsin, beta-glucuronidase, RNase, and DNase activities in rat kidneys.
- Utilizing DOCA-salt and spontaneously hypertensive rat (SHR) models.
- Comparing enzyme activities between hypertensive and control rats, and across different SHR substrains.
Main Results:
- Hypertensive rats with renal vascular lesions showed incomplete renin suppression and increased cathepsin, RNase, and DNase activities.
- SHR rats exhibited higher renin and cathepsin activities than Wistar-Kyoto (WK) rats, with further differences in DNase and beta-glucuronidase activities observed in female SHR.
- SHRSP rats showed higher renin and cathepsin activities than SHRSR rats, while PRC was higher in SHRSP at 10 months.
Conclusions:
- Renin and lysosomal enzyme activities are altered in hypertensive rats with renal vascular lesions.
- These enzymatic changes may play a significant role in the development and progression of hypertensive renal pathology.