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Sample stacking in laboratory-on-a-chip devices.

H Yang1, R L Chien

  • 1Caliper Technologies Corporation, Mountain View, CA 94043, USA.

Journal of Chromatography. A
|August 28, 2001
PubMed
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Field-amplified sample stacking concentrates samples in microfluidic devices. This new technique achieves hundreds of fold increases in sample concentration, integrating stacking with electrophoretic separation on a single chip.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Sample stacking is a crucial concentration technique widely used in capillary electrophoresis (CE).
  • Field-amplified sample stacking (FASS) is a popular method relying on electric field discontinuities for analyte concentration.
  • Existing microfluidic stacking methods face challenges in controlling the precise location of the stacked sample.

Purpose of the Study:

  • To present a novel sample stacking technique specifically designed for microfluidic laboratory-on-a-chip devices.
  • To achieve significant sample concentration increases using the new technique.
  • To integrate sample stacking with electrophoretic separation within a single microfluidic device.

Main Methods:

  • Development of a new sample stacking strategy for microfluidic devices.

Related Experiment Videos

  • Implementation of strategies to create electric field discontinuities for analyte stacking.
  • Integration of the developed stacking technique with on-chip electrophoretic separation.

    • Main Results:

    • Achieved up to hundreds of fold increases in sample concentration.
    • Demonstrated successful integration of sample stacking and electrophoretic separation in a single microfluidic device.
    • The new technique offers improved control over sample stacking in microfluidic systems.

    Conclusions:

    • The presented sample stacking technique significantly enhances sample concentration in microfluidic devices.
    • This method enables the combination of sample stacking and electrophoretic separation on a single lab-on-a-chip.
    • The technique holds promise for improving the sensitivity and efficiency of microfluidic analytical systems.