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Bonded dimethylacrylamide as a permanent coating for capillary electrophoresis
1Department of Chemistry, Karlsrad University, Sweden.
Journal of Chromatography. A
|August 28, 2001
Summary
Chemically bonded polydimethylacrylamide coatings for capillary electrophoresis (CE) offer high separation efficiency and stability. These new CE capillaries demonstrate excellent performance in separating diverse biomolecules.
Area of Science:
- Analytical Chemistry
- Separation Science
- Chromatography
Background:
- Capillary electrophoresis (CE) is a powerful separation technique.
- Capillary coatings are crucial for improving separation efficiency and stability in CE.
- Polydimethylacrylamide (PDMA) is a polymer with potential for capillary coating.
Purpose of the Study:
- To develop a method for coating capillaries with chemically bonded polydimethylacrylamide.
- To evaluate the properties and performance of these coated capillaries for CE applications.
Main Methods:
- Chemical bonding of polydimethylacrylamide to capillary inner surfaces.
- Evaluation of separation efficiency using cytochrome c.
- Assessment of electroosmotic flow (EOF) at different pH levels.
- Testing capillary stability at high pH.
- Demonstration of separation capabilities with various biomolecules.
Main Results:
- High separation efficiency achieved, with 1.2 x 10^6 theoretical plates/m for cytochrome c.
- Stable electroosmotic flow (EOF) observed, ranging from 10 x 10^-10 to 6 x 10^-10 m^2 V^-1 s^-1 at pH 8.0.
- Excellent capillary stability demonstrated, with over 150 runs possible at pH 10 without significant performance degradation.
- Successful separation of diverse analytes, including basic and acidic proteins, derivatized amino acids, and peptides.
Conclusions:
- Chemically bonded polydimethylacrylamide coatings provide a robust and efficient solution for capillary electrophoresis.
- The developed coated capillaries exhibit high separation performance and remarkable stability, making them suitable for complex biological sample analysis.
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