Related Experiment Video
Updated: Sep 10, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Simple and rapid high-performance liquid chromatography method for the determination of ofloxacin concentrations in
C Immanuel1, A K Hemanth Kumar
1Tuberculosis Research Centre, Chennai, India. trcicmr@md3.vsnl.net.in
Abstract:
A high-performance liquid chromatographic method for the determination of ofloxacin in human plasma and urine was developed. The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase C18 column and fluorescence detection at an excitation wavelength of 290 nm and an emission wavelength of 460 nm. The assay was linear from 0.5 to 10.0 microg/ml. The relative standard deviation of intra- and inter-day assays was lower than 5%. The average recovery of ofloxacin from plasma was 93%. The method was evaluated in samples from healthy subjects whose drug levels were already measured by microbiological assay.
Related Concept Videos
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Types of Detectors
Drug Concentrations: Measurements
Plasma —...
One-Compartment Open Model: Urinary Excretion Data and Determination of k
Measurement of Bioavailability: Pharmacokinetic Methods

