Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane cholesterol modulates serotonin transporter activity.

S M Scanlon1, D C Williams, P Schloss

  • 1Biochemistry Department, Trinity College, Dublin 2, Ireland.

Biochemistry
|August 29, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selected nitrostyrene compounds demonstrate potent activity in chronic lymphocytic leukaemia cells, including those with poor prognostic markers.

Oncology reports·2019
Same author

Suspected malignant hyperthermia in a horse anaesthetized with halothane.

Veterinary anaesthesia and analgesia·2017
Same author

Clinical Implications and Hospital Outcome of Immune-Mediated Myositis in Horses.

Journal of veterinary internal medicine·2017
Same author

Pre-clinical evaluation of a novel class of anti-cancer agents, the Pyrrolo-1, 5-benzoxazepines.

Journal of Cancer·2016
Same author

Survival of a Foal with Type A Botulism.

Journal of veterinary internal medicine·2016
Same author

Qualitative and Quantitative Characteristics of the Electroencephalogram in Normal Horses during Administration of Inhaled Anesthesia.

Journal of veterinary internal medicine·2015
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

Membrane cholesterol modulates serotonin transporter (SERT) function. Lowering cholesterol reduces SERT activity by decreasing substrate binding affinity and transport rate, highlighting cholesterol's crucial role in transporter stability.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Cell Biology

Background:

  • Serotonin transporter (SERT) regulates synaptic serotonin levels.
  • Cholesterol is a key component of cell membranes and influences protein function.

Purpose of the Study:

  • To investigate the role of membrane cholesterol in modulating serotonin transporter (SERT) activity.
  • To understand how cholesterol levels affect SERT's substrate and ligand binding affinity and transport rate.

Main Methods:

  • Stable expression of rat SERT in human embryonic kidney 293 cells.
  • Alteration of cellular cholesterol levels using methyl-beta-cyclodextrin (MbetaCD), cholesterol oxidase, and filipin.
  • Analysis of SERT activity, substrate binding, and ligand binding affinity.
  • Restoration of cholesterol levels using MbetaCD-cholesterol complexes and evaluation of functional recovery.

Related Experiment Videos

Main Results:

  • Cholesterol depletion significantly decreased SERT activity, reducing both substrate/ligand binding affinity and maximal transport rate.
  • Restoring cholesterol levels with MbetaCD-cholesterol complexes reversed the decrease in SERT activity and binding affinity.
  • Substitution with other steroids (ergosterol, 5-cholestene, pregnenolone) did not restore SERT function.

Conclusions:

  • Membrane cholesterol specifically modulates SERT functional properties.
  • Cholesterol is essential for stabilizing the serotonin transporter in its optimal active conformation.
  • Specific molecular interactions between cholesterol and SERT are critical for transporter function.