Related Experiment Video

Updated: Jul 16, 2026

Preparing T Cell Growth Factor from Rat Splenocytes
18:27

Preparing T Cell Growth Factor from Rat Splenocytes

Published on: October 31, 2007

DA rat NK(+)CD3(-) cells inhibit autoreactive T-cell responses

N A Wolf1, R H Swanborg

  • 1Department of Immunology and Microbiology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA.

Journal of Neuroimmunology
|August 30, 2001
PubMed

We isolated natural killer (NK) cells from DA rat bone marrow (BM) by staining with PE anti-NKR-P1A and FACS sorting (>98% NK(+)). The purified NK cells inhibit T-cell proliferation in a dosage-dependent fashion and suppressed production of the proinflammatory Th1 cytokine, IFN-gamma. When activated in vitro with Con A supernatant (CAS), the purified NK cells secrete the beta-chemokine monocyte chemoattractant protein-1 (MCP-1) and upregulate MCP-1 mRNA. The activated NK cells also express IFN-gamma mRNA. Sorted NK(+)CD3(-) cells, from which NKT cells have been excluded, also inhibit autoreactive T-cell responses to myelin basic protein (MBP). These findings are consistent with a role for conventional NK cells in maintaining immune homeostasis, by eliminating autoreactive T cells that have inadvertently become activated.

More Related Videos

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients18:48

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...