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Updated: Oct 4, 2026

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
Published on: January 31, 2025
Diversity and distribution in hypersaline microbial mats of bacteria related to Chloroflexus spp
U Nübel1, M M Bateson, M T Madigan
1Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, Montana, USA. unuebel@dsmz.de
Abstract:
Filamentous bacteria containing bacteriochlorophylls c and a were enriched from hypersaline microbial mats. Based on phylogenetic analyses of 16S rRNA gene sequences, these organisms form a previously undescribed lineage distantly related to Chloroflexus spp. We developed and tested a set of PCR primers for the specific amplification of 16S rRNA genes from filamentous phototrophic bacteria within the kingdom of "green nonsulfur bacteria." PCR products recovered from microbial mats in a saltern in Guerrero Negro, Mexico, were subjected to cloning or denaturing gradient gel electrophoresis and then sequenced. We found evidence of a high diversity of bacteria related to Chloroflexus which exhibit different distributions along a gradient of salinity from 5.5 to 16%.
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