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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Quantitative comparisons of 16S rRNA gene sequence libraries from environmental samples

D R Singleton1, M A Furlong, S L Rathbun

  • 1Department of Microbiology, University of Georgia, Athens, Georgia 30602-2605, USA.

Applied and Environmental Microbiology
|August 30, 2001
PubMed

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...