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Related Experiment Videos

Francisella tularensis strain typing using multiple-locus, variable-number tandem repeat analysis.

J Farlow1, K L Smith, J Wong

  • 1Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640, USA.

Journal of Clinical Microbiology
|August 30, 2001
PubMed
Summary

A new multilocus variable-number tandem repeat analysis (MLVA) system effectively types Francisella tularensis, the cause of tularemia. This method aids in tracking outbreaks and understanding the pathogen

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Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Francisella tularensis is the causative agent of tularemia, a zoonotic disease prevalent in the Northern Hemisphere.
  • Accurate typing methods are crucial for understanding the epidemiology and genetic diversity of F. tularensis.

Purpose of the Study:

  • To develop and evaluate a multilocus variable-number tandem repeat (MLVA) typing system for Francisella tularensis.
  • To assess the utility of MLVA for differentiating F. tularensis isolates and identifying outbreak sources.

Main Methods:

  • Genomic sequence analysis of F. tularensis to identify potential variable-number tandem repeat (VNTR) loci.
  • Development of PCR assays for six VNTR loci.
  • Typing of 56 F. tularensis isolates from various US states and the live vaccine strain.

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  • Cluster analysis to evaluate genetic relationships and compare with existing biovar classification.

    • Main Results:

    • The MLVA system detected variation at six VNTR loci, with allele numbers ranging from 2 to 20.
    • Cluster analysis revealed two genetically distinct groups, aligning with the current biovar classification of F. tularensis.
    • Biovar B isolates exhibited less genetic diversity compared to biovar A isolates.
    • Seven isolates from a recent Arizona epizootic were identical across all VNTR loci, suggesting a common infection source.

    Conclusions:

    • The developed VNTR markers are effective for identifying F. tularensis isolates at the taxonomic level of biovars.
    • MLVA is a valuable tool for rapid characterization and identification of F. tularensis outbreak isolates.
    • Future development of reference databases using MLVA will enhance outbreak tracking and global genetic relationship studies.